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Meena, Shashi
- Morphological and Molecular Characterization of Forensically Important Blow Fly Chrysomya megacephala (F.)
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Authors
Affiliations
1 Department of Zoology, University of Rajasthan, Jaipur 302004, Rajasthan, IN
2 Insect Molecular Biology Laboratory, Department of Entomology, Punjab Agricultural University, Ludhiana 147021, Punjab, IN
1 Department of Zoology, University of Rajasthan, Jaipur 302004, Rajasthan, IN
2 Insect Molecular Biology Laboratory, Department of Entomology, Punjab Agricultural University, Ludhiana 147021, Punjab, IN
Source
Indian Journal of Entomology, Vol 84, No 2 (2022), Pagination: 301-306Abstract
Considering the significance of Chrysomya megacephala (F.) (Diptera: Calliphoridae) and their larval instars in forensic-entomological investigations, a culture of C. megacephala was established and morphological features of second and third larval instars were studied under a compound microscope. To validate the species’ morphological identification, sequencing of the ‘standard barcode region’ (658 base pairs of mitochondrial cytochrome oxidase subunit I gene) was performed and this sequence was subjected to BLAST analysis using the NCBI database which acknowledged it as Chrysomya megacephala with 99.39% identity with sequence of same species collected from Maharashtra, India. The phylogenetic analysis congregated Jaipur population separately than other samples from different regions of India, however, not much variations were recorded. This is the maiden report of molecular identification of C. megacephala from Rajasthan.Keywords
Chrysomya megacephala, Diptera, morphology, adult, maggots, DNA barcoding, BLAST, ClustalW, phylogenetic analysisFull Text
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